PDF | Heliopsis longipes is an herbaceous plant found in Mexico. Heliopsis longipes is traditionally used for its analgesic and anesthetic properties. PDF | Heliopsis longipes S.F. Blake (Asteraceae: Heliantheae) (chilcuague) is used in Mexican traditional medicine against parasites and to alleviate tooth and . Heliopsis longipes (A. Gray) Blake (Asteraceae) is a species broadly used in Mexican Traditional Medicine. The present study illustrates the.
|Language:||English, Portuguese, Dutch|
|Genre:||Children & Youth|
|ePub File Size:||24.42 MB|
|PDF File Size:||10.58 MB|
|Distribution:||Free* [*Register to download]|
Keywords: Heliopsis longipes, affinin, vasodilation, rat aorta, Available online : ciofreedopadkin.cf Bauer R. Heliopsis longipes S.F. Blake (Asteraceae) is a perennial herb endemic to Sierra Gorda and Sierra Alvarez, a moun- tainous region located in. ABSTRACT. Heliopsis longipes is a popular medicinal plant in Mexico. One of the main constituents that can be extracted from H. longipes is affinin.
Constituents of Heliopsis Species. IV. The Total Synthesis of trans
In addition, alcohol extracts com. DOI: The total yield of pure affinin was Affinin yield with respect to extract weight. The purity of affinin We therefore studied the neuropharma- using NMR and comparison of its retention time cological profile of the plant extract with the present The H.
All experi- Aldrich Co. Efforts were made to minimize animal Mexico and diazepam Valium were used in this suffering and to reduce the number of animals used. Each animal was tested once.
At administered in a volume of 0. For nociception pretreatment, the H. A boxymethylcellulose in 0. Antinociceptive effect The procedure performed was similar to that pre- viously described by Wheeler-Aceto and Cowan . They were then removed for formalin The extraction solvent was concentrated to dryness administration. The isolation of the dorsal surface of the right hind paw with a g Drug Dev.
The holes provided a immediately observed after formalin injection.
Purely olefinic alkamides in Heliopsis longipes and Acmella (Spilanthes) oppositifolia
A mirror measure of the number of head dips. Nociceptive behavior was quantified as the numbers of flinches of the injected paw during 1-min Exploratory rearings intervals every 5 min up to 60 min after injection. Each mouse was individually placed on a filter Flinching was readily identified and was characterized paper-covered floor in a glass cylinder 16 cm in height as rapid and brief withdrawal or flexing of the injected and 11 cm in diameter.
The number of spontaneous paw. Formalin-induced flinching behavior is biphasic, rearings on its posterior limbs was counted during the with the initial acute phase 0—10 min being followed first 5 min, and a reduced number of exploratory rear- by a relatively short quiescent period, which is then ings indicated an anxiolytic effect.
The rats received an equivalent dose of vehicle 50 ml or increasing doses of the H. The area under the number of flinches against 40 cm in height.
The number of entries into any of the time curve, an expression of the duration and intensity arms and the total time spent in each of the two arm types of the effect, was calculated using the trapezoidal rule. The total number of entries into the CNS evaluation arms provided a measure of the general activity. The procedure for CNS activity was performed in the male mice.
Sodium PB-induced hypnosis that began 5 days before evaluation of the H. Each mouse was observed for the onset of unco- control for each experimental procedure. The groups ordinated motor behavior sedative phase , loss of right- were composed of seven mice. Ambulatory activity, ing reflex hypnosis , and duration of sleep.
The criterion antianxiety effects the elevated plus-maze, hole board for sleep was defined as the loss and recovery of the test, and exploratory rearings , sodium PB-induced righting reflex, which was recorded as the sleep time. The latency for onset of the generalized clonic Fifty minutes after the administration of vehicle, and tonic seizures was evaluated for 30 min after PTZ diazepam, the H. The control animals were administered with were placed one by one inside a cage that was divided vehicle.
The number of squares the min time limit, the animals were considered to explored by each mouse in a 2 min interval was be protected from PTZ-induced seizures. This mouse con- tinued with the antianxiety test. Food was withheld for 12 h prior to any experi- Mice were individually placed in the center of a ments with animals having free access to drinking perforated board and the number of head dips was water.
The concentration of the tested compounds was registered during a 3-min trial. The perforated board adjusted for oral administration of 0.
The mice were treated in two phases. In the Drug Dev. IL of crude extract were administered three animals per administration of the H. For the paw and affinin 0. In both phases, the mice were phases Fig. When the H. CL paw, the antinociceptive effect was similar to IL administration Fig.
As shown in Table 1, the H. Anxiety was tested in the with agitation 90 rpm in an American Scientific-BT23 open-side arms, head dipping, and rearing tests. The water bath, Toronto, Canada.
After growth, ml of H. The with 2 ml of soft agar that contained histidine-biotin antianxiety effect of the extract resulted in approxi- 0. Affinin administration decreased the number described by Maron and Ames . The number of kg, i.
Therefore, part of the antianxiety effects of the histidine reversion colonies was determined using a H. Diazepam Fisher colony counter. Positive decreased the time in the open-side arms and controls for TA98, TA, TA without the enzymatic decreased head dipping and rearing see Table 1. Posi- PB-induced sedative-hypnotic effects with respect to tive controls employed in the presence of the S-9 vehicle administration in a dose-dependent manner. These results suggest that the H.
Statistical analysis The H. All doses of ].
Statistical differences were analyzed using one- the H. Diazepam prevented the convul- sions and no animals died on its administration. The first phase Toxicological Assays began immediately after administration of formalin and The lethal dose 50 LD50 study was performed to diminished gradually after approximately 10 min. Local antinociceptive effect of Heliopsis longipes extract and affinin during the formalin test.
The rats received H. Data are expressed as the area under the number of flinches against time curve AUC. TABLE 1. Drug Dev. TABLE 3. The criterion for sleep was defined as the loss and recovery of the righting reflex, which was recorded as the sleep time.
The latency for onset of the generalized clonic and tonic seizures was evaluated for 30 min after PTZ injection. The control animals were administered with vehicle. If no convulsions or mortality occurred during the min time limit, the animals were considered to be protected from PTZ-induced seizures. Food was withheld for 12 h prior to any experiments with animals having free access to drinking water.
The concentration of the tested compounds was adjusted for oral administration of 0. The mice were treated in two phases. Ames test The mutagenicity tests were performed using the tube incorporation method described by Maron and Ames  and Dciga-Campos et al.
After growth, ml of each strain was transferred to sterile screw-top tubes with 2 ml of soft agar that contained histidine-biotin 0. The total tube contents were spread onto plates with VogelBonner media and were incubated for 48 h at 37C. All experiments were performed in triplicate.
The number of histidine reversion colonies was determined using a Fisher colony counter. The reversion rate was compared with control plates with and without mutagens.
Statistical analysis All results are presented as a mean of seven animals SEM except in the toxicological studies where three animals were used per group [Lorke, ]. The first phase began immediately after administration of formalin and diminished gradually after approximately 10 min.
The second phase began after 15 min and lasted for 1 h. IL administration of the H. When the H. As shown in Table 1, the H. Anxiety was tested in the open-side arms, head dipping, and rearing tests. The H.
Therefore, part of the antianxiety effects of the H. Affinin induce a similar behavior to vehicle administration Table 2. These results suggest that the H.The solution turned cloudy, a white solid separated, and the temperature of the mixture rose gradually t o 76'. Langfelder, K. Rai, M. Effect of essential oils on the growth of Fusarium verticillioides and fumonisina contamination in corn.
Vargas-Arispuro, I. Each mouse was observed for the onset of uncoordinated motor behavior sedative phase , loss of righting reflex hypnosis , and duration of sleep.